Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
9.4K Views
•
10:58 min
•
March 29th, 2017
DOI :
March 29th, 2017
•Graham Brogden*1,2, Ariane Neumann*1,3, Diab M. Husein1, Friederike Reuner1,4, Hassan Y. Naim1, Maren von Köckritz-Blickwede1,4
1Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 2Fish Disease Research Unit, University of Veterinary Medicine, 3Department of Clinical Sciences, Biomedical Center, Lund University, 4Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover
Chapters in this video
0:05
Title
1:14
Isolation of Human Blood-derived Neutrophils by Density Gradient Centrifugation
3:59
Isolate the Lipids from the Human Peripheral Blood-derived Neutrophils
5:33
High Performance Thin Layer Chromatography (HPTLC) to Semi-quantify the Amount of Lipids
7:53
High Performance Liquid Chromatography (HPLC) to Quantify the Amount of Cholesterol
8:48
Results: Isolation of Human Blood-derived Neutrophils and Subsequent Lipid Analysis
9:33
Conclusion
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