Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
7.4K Views
•
10:34 min
•
February 22nd, 2017
DOI :
February 22nd, 2017
•Muthukumar Balasubramaniam1,2, Benem Davids1,2, Amma B. Addai1,2, Jui Pandhare1,3,4, Chandravanu Dash1,2,5
1Center for AIDS Health Disparities Research, Meharry Medical College, 2Department of Biochemistry and Cancer Biology, Meharry Medical College, 3School of Graduate Studies and Research, Meharry Medical College, 4Department of Microbiology and Immunology, Meharry Medical College, 5Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College
Chapters in this video
0:05
Title
1:36
Virus Infection of Sup-T1 Cells
3:22
Harvesting and Lysis of Infected Cells
4:22
Preparation of Cytoplasmic Preintegration Complexes
5:32
In Vitro Integration Assay Using Preintegration Complexes
7:40
PCR Assays for Quantifying Preintegration Complex Activity
8:29
Results: HIV-1 Preintegration Complex-associated vDNA Integration Activity
9:47
Conclusion
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