Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
12.7K Views
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08:40 min
•
April 8th, 2016
DOI :
April 8th, 2016
•1Division of Urologic Surgery, Washington University in St. Louis School of Medicine, 2Department of Urology, University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health
Chapters in this video
0:05
Title
0:37
Automated Image Acquisition and Analysis
1:43
Tissue Segmentation
3:48
Cell Segementation
5:16
Scoring and IHC and Co-localization
5:50
Applying the Algorithm and Batch Analysis
7:08
Results: Successful Cell and Tissue Segmentation
8:18
Conclusion
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