Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
10.1K Views
•
10:38 min
•
September 9th, 2015
DOI :
September 9th, 2015
•Audrey Bernut1,2, Christian Dupont1,2, Alain Sahuquet1, Jean-Louis Herrmann3, Georges Lutfalla1, Laurent Kremer1,2
1Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, UMR 535, Université Montpellier, 2Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé, CNRS, FRE 3689, Université Montpellier, 3Unité de Formation et de Recherche des Sciences de la Santé, EA3647-EPIM, Université Versailles St Quentin
Chapters in this video
0:05
Title
1:35
Preparation of M. abscessus Inocula
3:20
Microinjection of Zebrafish Embryos
5:25
Generation of Macrophage-depleted Embryos
6:13
Bacterial Burden Quantification
7:16
Imaging M. abscessus-infected Embryos
8:34
Results: M. abscessus Infection in Zebrafish Embryos
10:03
Conclusion
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