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Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

42.6K Views

12:09 min

May 2nd, 2011

DOI :

10.3791/2444-v

May 2nd, 2011


Transcript

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Recombinant RBD based SARS Vaccines

Chapters in this video

0:05

Title

1:01

Introduction

1:52

Recombinant SARS-CoV RBD Protein Preparation

4:40

Mouse Immunization and Sample Collection

5:56

Preparation of SARS Pseudovirus in 293T Cells

6:53

SARS Pseudovirus Titration and Neutralization Assay

11:10

Evaluation of Recombinant RBD-induced Neutralizing Activity

11:36

Conclusion

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