CRISPR/Cas9 Edição do Gene C. elegans rbm-3.2 utilizando o marcador de triagem co-CRISPR dpy-10 e complexos de ribonucleoproteína montados.

0:53

C. elegans Gonad Preparation and Microinjection

1:56

Injected Worm Recovery and Transfer

2:37

PickingC. elegans for Screening

3:17

Single Worm Lysis and PCR

4:22

Restriction Digestion and Agarose Gel Electrophoresis

5:08

Identification of Positively Edited and Homozygous Edited Worms

6:01

Results: Representative C. elegans rbm-3.2 Gene Editing and Screening

7:10

Conclusion