Hi everyone. My name is Mean Jing, and I'm from USTC. As we know that the Opto kinetic response, or OKR for short, is a behavior test that an animal moves its eyes to follow a grading.
Moving around it. This behavior has been widely used. Assess the visual functions of larval zebra fish.
However, the method using the standard prodigal to fix the larval fish is not readily applicable for adults. So in this video, we're gonna show you a new prodigal that makes it possible to record the adult OKR. And also for comparison, we will demonstrate the OKR test for larva fish and the OMR test for adult fish.
And by OMR, I mean motor response. Now I will show you our customized OKR app appearance. It looks nice, huh?
In this a appearance, there's a grating base driven by a motor. The grating tube rests on this grating base and rotates with it. A chamber plate is placed over the grating base, but does not rotate, and a circular lamp is used to lit the grating.
And that's it. Just put the fish chamber on the chamber plate and we're ready to roll. Hi, I'm Sui.
Hi, I'm W.We are working in the neur development and the ELL of USPC. Today we'll show you sweep the high test of the zebrafish. Okay, let's start.
First of all, we're gonna show you how we did the OKR test for adult zebrafish. Now put the sponge into the fish chamber and pour some water into it. Squeeze the sponge a little bit to get rid of the air bubbles.
Now we're gonna show you how to fix the fish With pins step by step. Now let's wiggle the fish a little bit to make sure his dorsal hin points upward and stretch the tail to make the body straight. Use the artery clamp to fix the tail fin and stick the clamp to the chamber by using the modeling Clay.
After the fixing, pour more Water into the chamber and blow some water towards the fish to awake it. Now we're finally done with the preparation And ready for the real adventure. That's the OKR test.
Note that the two objects in the upper part of the screen are merely the reflection of the grating on the pinhead. Now let's see how we did the larva OKR test. We put the male and female Fish in the mating tank and let them lay eggs.
Then we collect the eggs and transfer them to a glass dish. We use meth cellose Solution to fix the larva fish. The solution must be cooled down quickly after being heated.
So we put it in ice water and gently shake it until it becomes transparent. Now put 20 milliliter methylcellulose in a dish. Make sure that There is no air bubble in the solution.
Put the larvae in a row in the solution. Add a tiny one microliter air bubble in the view so that you Can see the reflection of the grading. That's the Demonstration of the OKR behavior of wild type zebra fish larvae.
In the end, we're gonna show you how to do the OMR test for adult zebra fish. First of all, catch a fish And put it in a fish chamber. Now put the fish chamber in place.
Then install a white cylinder at the center of the chamber so that fish Cannot pass the center before the test. It is better to let the fish adept in this lighting condition for one minute. Okay, that's the demonstration of the OMR test for adult zebra fish.
We just showed you how To perform the OKR test on adult zebra fish. In this test, we made an attempt to fix the zebra fish with pins instead of methylcellulose. Using this method, we managed to record a strong response that lasted over four hours.
That will give the experimenters Plenty of time.
