JoVE Logo

Zaloguj się

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

402 Views

06:17 min

February 28th, 2025

DOI :

10.3791/67178-v

February 28th, 2025


Przeglądaj więcej filmów

Covalent Fragment Screening

Rozdziały w tym wideo

0:00

Introduction

1:16

Quantitative-Irreversible Tethering Assay for Electrophilic Ligand Screening

2:48

CPM-Quenching to Quantify the Amount of Unreacted Thiol Remaining in the Protein and Glutathione Reaction Plates of Quantitative-Irreversible Tethering Assay

4:18

Data Analysis and Hit Selection After the Quantitative-Irreversible Tethering Assay for Electrophilic Ligand Screening

Powiązane Filmy

article

10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

8.5K Views

article

11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

12.8K Views

article

11:13

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

11.0K Views

article

07:23

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

8.5K Views

article

09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

1.8K Views

article

06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

5.3K Views

article

09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

3.1K Views

article

06:26

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

4.7K Views

article

08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

4.9K Views

article

07:22

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

3.2K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone