A Silicosis Mouse Model Established by Repeated Inhalation of Crystalline Silica Dust
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2.1K Views
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10:45 min
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January 6th, 2023
DOI :
January 6th, 2023
•Hangbing Cao*1,2,3,4, Bing Li*1,2,3,4, Haoming Chen1,3,4, Yehong Zhao1,4, Yuanjie Zou1,4, Yang Liu1,4, Min Mu1,2,3,4, Xinrong Tao1,2,3,4
1Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, 2Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, 3Anhui Province Engineering Laboratory of Occupational Health and Safety, 4School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology
Rozdziały w tym wideo
0:04
Introduction
1:07
Preparing the Crystalline Silica Dust (CSD) Suspension
1:52
Administering the Nasal Drips to the Mouse
2:24
Performing Hematoxylin and Eosin (HE) Staining
3:23
Performing Masson Staining and Sirius Red Staining
4:27
Performing Immunohistochemistry
6:10
Performing Western Blotting Analysis
8:02
Results: Investigating the Development of Silicosis in Mice After Repeated CSD Inhalation
9:41
Conclusion
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