Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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2.2K Views
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04:52 min
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July 1st, 2022
DOI :
July 1st, 2022
•Sriram Aiyer1, Timothy S. Strutzenberg1, Marianne E. Bowman2, Joseph P. Noel2,3, Dmitry Lyumkis1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Rozdziały w tym wideo
0:05
Introduction
0:28
Setting Up the Tilted Data Collection
3:23
Results: Images of the Grid at Square Magnification with Different Tilt Angles and Hole Exposures and 2D Class Averages Collected at Different Tilt Angles
4:27
Conclusion
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