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Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

3.5K Views

07:59 min

June 29th, 2021

DOI :

10.3791/62628-v

June 29th, 2021


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Protein Aggregates

Rozdziały w tym wideo

0:05

Introduction

0:53

Stress Treatment of E. coli Strains MG1655 and CFT073

2:12

Collecting Bacterial Cell Samples

2:43

Extracting the Insoluble Protein Aggregates

4:19

Soluble Protein Sample Preparation

5:30

Separation and Visualization of Extracted Protein Aggregates Using SDS-PAGE

6:49

Results: Visualization of Protein Aggregates After Treatment of Escherichia coli with a Proteotoxic Stressor

7:32

Conclusion

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