Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
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3.3K Views
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11:38 min
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September 14th, 2021
DOI :
September 14th, 2021
•Rozdziały w tym wideo
0:05
Introduction
0:40
Cell Culture and Fluid Shear Stress Exposure
2:55
Fixation
4:58
Blocking and Incubation with Primary Antibody
6:28
Incubation with PLA Probe and Ligation
7:34
Amplification and Mounting
9:14
Results: Effect of Shear Stress on the SMAD Protein Interactions
11:02
Conclusion
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