Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
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7.9K Views
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09:10 min
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July 9th, 2019
DOI :
July 9th, 2019
•Rozdziały w tym wideo
0:04
Title
1:16
CAS9-gRNA Ribonucleoprotein Mixture and Agarose Plate Preparation
2:33
Electroporator Configuration and Glass Microinjection Capillary Loading
4:09
Pneumatic Pump Configuration
4:37
CAS9-gRNA Ribonucleoprotein Mixture Injection
6:05
Electroporation
6:45
Knock-Out Efficiency Assessment
7:25
Results: Representative Knock-Out Effects in Axolotl Spinal Cord Neural Stem Cells
8:33
Conclusion
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