Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10.7K Views
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10:33 min
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June 17th, 2019
DOI :
June 17th, 2019
•Rozdziały w tym wideo
0:04
Title
0:53
Bacterial Cultivation and Extraction of Genomic DNA
1:54
Multiplex PCR Setup and Cycling Conditions
3:14
PCR Amplicon Confirmation by Gel Electrophoresis
4:37
Capillary Electrophoresis Setup and Run Conditions
6:06
VNTR (Variable-number Tandem-repeat) Size Calling, Repeat Count Calculation and MLVA (Multi-locus Variable-number Tandem-repeat Analysis) Profiling
7:36
Minimum Spanning Tree Cluster Analysis of MLVA Data
9:21
Results: Gel Electrophoresis, Electropherograms, and Minimum Spanning Tree
10:09
Conclusion
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