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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

7.4K Views

11:20 min

August 30th, 2017

DOI :

10.3791/55769-v

August 30th, 2017


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Upconversion Nanoparticles

Rozdziały w tym wideo

0:05

Title

1:33

Fluorimeter Setup for Upconversion Spectrum Measurement and Microscope Setup

9:40

Conclusion

8:33

Results: Visualize PKA Signal Transduction Activation via NIR Photoactivation

3:29

Characterization of Caged-PKA and Caged PKA/UCNP Constructs and Photolysis Setup

4:55

Cell Sample Preparation and Microinjection of Caged-PKA/UCNP Complexes

7:35

Photoactivation of Caged-PKA/UCNP Using NIR Light in Living Cells Experiment

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