Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
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6.7K Views
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09:36 min
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August 13th, 2017
DOI :
August 13th, 2017
•Rozdziały w tym wideo
0:00
Title
0:33
Preparation of Buffers and Reagents
0:38
Preparing p-Nitrophenol Phosphate (pNPP) Buffer
1:21
Preparing Malachite Green Solution
2:21
Preparation of Activator
2:42
Preparing Malachite Green + Tween 20 (MGT) Working Solution
2:58
Preparing Threonine Phosphopeptide (pT)
3:31
Synuclein Preparation
3:59
Preparing Phosphate Standards
4:45
Phosphate Standards Step 2
5:17
PP2A Activity Assay
6:42
Photometric Analysis
6:53
Representative Results
9:01
Conclusion
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