Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
Please note that all translations are automatically generated. Click here for the English version.
11.4K Views
•
09:53 min
•
February 6th, 2017
DOI :
February 6th, 2017
•Rozdziały w tym wideo
0:05
Title
1:05
T-cell Receptor (TCR) and Human Leukocyte Antigen-resticted Tumor-derived Peptide (pHLA) Refolding and Purification by Fast Protein Liquid Chromatography (FPLC)
3:43
Surface Plasmon Resonance (SPR) Equilibrium Binding and Thermodynamic Analyses
5:55
Protein Crystallization and X-ray Diffraction
7:39
Results: Molecular Analysis of gp100280-288 Recognition by CD8+ T-cells
8:59
Conclusion
Powiązane Filmy
11:31
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
24.9K Views
16:10
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
23.8K Views
08:04
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
11.6K Views
09:28
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
14.9K Views
10:31
Real-time Live Imaging of T-cell Signaling Complex Formation
13.6K Views
07:36
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
5.2K Views
09:24
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
7.9K Views
19:05
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
12.3K Views
13:19
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
2.5K Views
08:49
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
9.1K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone