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High-throughput Titration of Luciferase-expressing Recombinant Viruses

12.8K Views

08:09 min

September 19th, 2014

DOI :

10.3791/51890-v

September 19th, 2014


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Keywords High throughput

Rozdziały w tym wideo

0:05

Title

1:16

Sample Preparation and Preparation of Permissive Cells for Virus Titration

2:57

Preparation of Viral Standard Curve and Transfer of Sample Supernatants on to Permissive Cells

4:08

Assessment of Cell Viability and Preparation of the Luciferin Substrate

4:53

Reading Bioluminescence and Data Analysis

5:46

Results: Estimated Viral Titers and Sample Cytotoxicity

7:01

Conclusion

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