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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

80.2K Views

12:40 min

October 20th, 2014

DOI :

10.3791/51805-v

October 20th, 2014


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Keywords Clathrin mediated Endocytosis

Rozdziały w tym wideo

0:05

Title

1:21

Expression of Flourescently Tagged CME Components in Cultured Cells

2:42

Imaging Clathrin-coated Pit (CCP) and Cargo Dynamics Using TIRFM

5:22

Analysis of Endocytic Dynamics by Manual Verification

6:32

Analysis of Endocytic Dynamics by Objective Recognition

10:40

Results: Anlysis of the Endocytic Dynamics of the μ‐opioid‐receptor (MOR), a GPCR, and its Endocytic Adaptor Protein β‐arrestin

12:18

Conclusion

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