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Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example

16.0K Views

14:20 min

October 8th, 2014

DOI :

10.3791/51652-v

October 8th, 2014


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Osmotic Water Permeability

Rozdziały w tym wideo

0:05

Title

2:10

Isolation of Arabidopsis Mesophyll Protoplasts

4:13

The Hypotonic Challenge Assay: Arabidopsis Mesophyll Cell Swelling

6:36

Analysis of Cell Volume Change Using ImageJ

8:12

Modling the Rate of Osmolarity Change in the Experimental Chamber Using ImageJ and the Matlab Program PfFit

11:11

Determining the Pf Using the Matlab Fitting Program PfFit

12:30

Results: Determination of the Osmotic Water Permeability Coefficient (Pf) in Plant Protoplasts

13:37

Conclusion

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