ツイン連鎖球菌タグタンパク質とのStrep-Tactinの樹脂ビスで架橋された（スルホスクシンイミジル）スベレート（BS3）に対するそれらの複合体のいずれかの段階精製

0:11

Introduction

1:32

Overview

1:37

Competitive Elution with Biotin

2:44

Denaturing Elution with SDS

3:26

Chemical Cross-linking of the Resin

4:10

Protocol

4:13

Cross-linking of the Resin with BS3

7:22

Quenching of the Cross-linking Reaction

9:50

Binding of the Bait Protein and Associated Complexes to the Resin

12:18

Washing of the Resin

14:23

Elution of Specific Protein Complexes

15:32

Representative Results

17:18

Conclusion