Developing an Experimental Aneurysm Model in a Rabbit Using an Elastase-Treated Vessel Pouch

Begin with an anesthetized rabbit with surgically exposed carotid arteries or CCAs supported by a blue pad for clear visualization.

Tie off the right CCA with sutures and clamp it distally to restrict blood flow.

Cut the section of the right CCA containing the tight knot to create a vessel pouch and clean it in heparinized saline to remove soft tissue.

Treat the pouch with a buffer containing elastase to degrade elastin fibers, weakening the vessel.

Support the left CCA and clip it to restrict blood flow.

Remove the attached soft tissues and pierce the left CCA to release trapped blood. 

Inject saline containing heparin to prevent blood clotting. 

Suture the cut right CCA to the left CCA. Later, ligate the elastase-treated pouch.

Now, remove all clamps to restore blood flow and suture the incision.

Blood flow in the artificial carotid bifurcation induces stress on the weakened vessel pouch walls, causing it to bulge and form an aneurysm.