JoVE Logo

サインイン

コンセプト実験

このコンテンツを視聴するには、JoVE 購読が必要です。

Modeling Transuterine Fetal Tracheal Occlusion in Murine Model: A Surgical Procedure for Ligation of the Fetal Trachea Within a Pregnant Mouse

-- views • 1:46 min

記録

Place an anesthetized pregnant mouse of desired gestation age in the supine position. Sterilize the abdominal area. Perform laparotomy—a surgical incision through the abdominal wall. Dissect the abdominal muscles to expose the abdominal cavity.

Identify the uterine horns—long tubular structures that house multiple fetuses—allowing them to develop simultaneously. Gently exteriorize the uterine horns from the abdominal cavity, positioning them in a transverse orientation. Further, visualize the neck of the candidate mouse embryo.

Gently insert a surgical needle with the suture of appropriate thickness transversely through the uterine horn region, away from the placenta. Advance the needle further to the anterior part of the neck where the trachea is located. Direct the needle to exit the neck opposite the uterine horn. Carefully tighten the suture to occlude the trachea, ensuring uterine wall integrity. Return the uterine horns into the abdominal cavity.

Inject warm saline into the peritoneal cavity to facilitate pregnancy progression. Close the surgical incision and allow the mouse to recover. The established mouse fetal tracheal occlusion model can be used to study the impact of occluded trachea on lung development.

article

02:10

Modeling Transuterine Fetal Tracheal Occlusion in Murine Model: A Surgical Procedure for Ligation of the Fetal Trachea Within a Pregnant Mouse

関連動画

1.0K Views

JoVE Logo

個人情報保護方針

利用規約

一般データ保護規則

研究

教育

JoVEについて

Copyright © 2023 MyJoVE Corporation. All rights reserved