The present protocol assesses the locomotor activity of Drosophila by tracking and analyzing the movement of flies in a hand-made arena using open-source software Fiji, compatible with plugins to segment pixels of each frame based on high-definition video recording to calculate parameters of speed, distance, etc.
The protocol introduces a novel mini-open TLIF, which can significantly reduce intraoperative blood loss, achieving minimally invasive outcomes with enhanced recovery.
Copyright © 2023 MyJoVE Corporation. All rights reserved