Developing Myopia in a Mouse Model Through Lens-Induced Defocusing

Start with an anesthetized mouse with an exposed skull bone.

Apply an etching agent to remove the periosteum and create a cleaner surface.

Position the eyeglass holder with lens frames so the eyes are centered within the frames.

Apply adhesive to the holder and let it set, securing it to the skull.

Remove the lens frames to minimize discomfort during recovery.

Intraperitoneally inject a recovery agent to recover the mouse.

When the mouse is awake, reattach the frame containing concave lenses covering the eyes.

These lenses disperse incoming light, causing it to converge behind the retina and resulting in blurred vision.

Retinal neurons detect the blur and signal the brain to elongate the eyeball to focus the light on the retina for clear vision.

After the lenses are removed, the elongated eyeball causes light to converge in front of the retina, impairing near vision in the mouse and resulting in myopia.