Abigail Wexner Research Institute at Nationwide Children's Hospital

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Iftekhar A. Showpnil^1,2, Kyle R. Miller¹, Cenny Taslim¹, Kathleen I. Pishas¹, Stephen L. Lessnick^1,3, Emily R. Theisen^1,4

¹Center for Childhood Cancer and Blood Diseases, Abigail Wexner Research Institute at Nationwide Children's Hospital, ²Molecular, Cellular, and Developmental Biology Program, The Ohio State University, ³Division of Pediatric Hematology/Oncology/Blood & Marrow Transplant, The Ohio State University, ⁴Department of Pediatrics, The Ohio State University

Intrinsically disordered domains are important for oncogenic fusion transcription factor function. To therapeutically target these proteins, a more detailed understanding of the regulatory mechanisms employed by these domains is required. Here, we use transcriptomics to map important structural features of the intrinsically disordered EWS domain in Ewing sarcoma.

Bioengineering

Wireless Telemetry Device Implantation in a Fontan Ovine Model for Continuous and Long-Term Hemodynamic Monitoring

Marissa Guo^1,2, Tatsuya Watanabe¹, Cameron DeShetler¹, Satoshi Yuhara¹, Jennifer Kievert^1,3, Logan Spiess¹, Adrienne Morrison¹, Kirsten Nelson¹, Eric Heuer¹, Fahd Taha¹, John M. Kelly^1,4, Christopher K. Breuer^1,5

¹Center for Regenerative Medicine, Abigail Wexner Research Institute at Nationwide Children's Hospital, ²Department of Surgery, The Ohio State University Wexner Medical Center, ³Biomedical Sciences Graduate Program, The Ohio State University College of Medicine, ⁴Department of Pediatric Cardiology, Nationwide Children's Hospital, ⁵Department of Pediatric Surgery, Nationwide Children's Hospital

This protocol describes the surgical methodology for implanting a large animal wireless telemetry device to enable continuous and long-term collection of hemodynamic data, including heart rate, arterial blood pressure, inferior and superior vena cava pressures, and cardiac rhythm.