UNSW Sydney

2 ARTICLES PUBLISHED IN JoVE

Medicine

Palatable Western-style Cafeteria Diet as a Reliable Method for Modeling Diet-induced Obesity in Rodents

Sarah-Jane Leigh^*1, Michael D. Kendig^*1, Margaret J. Morris¹

¹Department of Pharmacology, School of Medical Sciences, UNSW Sydney

This protocol describes the use of a highly palatable, western-style cafeteria diet to model overeating and obesity in rodents. Here, we provide a detailed outline of food selection, preparation and measurement, and explain methodological factors that assist in generating a robust and reproducible phenotype.

Cancer Research

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

Maria Tsoli^1,2, Yongjuan Chen¹, Anjana Gopalakrishnan¹, Caitlin Ung¹, Aaminah Khan^1,2, Dannielle Upton^1,2, David S. Ziegler^1,2,3

¹Children's Cancer Institute Australia, Lowy Cancer Research Centre, UNSW Sydney, ²School of Clinical Medicine, Faculty of Medicine & Health, UNSW Sydney, ³Kid's Cancer Centre, Sydney Children's Hospital

The development of orthotopic pediatric brain tumor models requires meticulous precision, using a stereotaxic device to implant cancer cells precisely. The methodology presented here outlines the steps involved in preparing brain tumor cells, performing intracranial injections, and implementing a post-operative monitoring system to assess brain tumor engraftment.