Sydney Children's Hospital

2 ARTICLES PUBLISHED IN JoVE

Medicine

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency

Katelin M. Allan^1,2, Sharon L. Wong^1,2, Laura K. Fawcett^1,2,3, Alexander Capraro^1,2, Adam Jaffe^1,2,3, Cristan Herbert⁴, Elvis Pandzic⁵, Shafagh A. Waters^1,2,3

¹School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, ²Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, ³Department of Respiratory Medicine, Sydney Children's Hospital, ⁴Department of Pathology, School of Medical Sciences, University of New South Wales Australia, ⁵Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales

This protocol describes nasal epithelial cell collection, expansion, and differentiation to organotypic airway epithelial cell models and quantification of cilia beat frequency via live-cell imaging and custom-built scripts.

Cancer Research

Establishment of Orthotopic Patient-derived Xenograft Models for Brain Tumors using a Stereotaxic Device

Maria Tsoli^1,2, Yongjuan Chen¹, Anjana Gopalakrishnan¹, Caitlin Ung¹, Aaminah Khan^1,2, Dannielle Upton^1,2, David S. Ziegler^1,2,3

¹Children's Cancer Institute Australia, Lowy Cancer Research Centre, UNSW Sydney, ²School of Clinical Medicine, Faculty of Medicine & Health, UNSW Sydney, ³Kid's Cancer Centre, Sydney Children's Hospital

The development of orthotopic pediatric brain tumor models requires meticulous precision, using a stereotaxic device to implant cancer cells precisely. The methodology presented here outlines the steps involved in preparing brain tumor cells, performing intracranial injections, and implementing a post-operative monitoring system to assess brain tumor engraftment.