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Sarishna Singh

Division of Molecular Biology and Human Genetics,
SAMRC Centre for TB Research,
DSI-NRF Centre of Excellence for Biomedical TB Research,
Department of Biomedical Sciences,
Faculty of Medicine and Health Sciences,
Division of Molecular Biology and Human Genetics, SAMRC Centre for TB Research, DSI-NRF Centre of Excellence for Biomedical TB Research, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences

Stellenbosch University

BIOGRAPHY

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PUBLICATIONS

Comparison of commercial assays and two-step approach to detect in South Africa.

African journal of laboratory medicine , 2022 | Pubmed ID: 36263391

Incorporating direct molecular diagnostics in management algorithms for nontuberculous mycobacteria: Is it high time?

IJID regions Mar, 2024 | Pubmed ID: 38304760

Misdiagnosis of non-tuberculous mycobacteria as tuberculous by the GeneXpert MTB/RIF Ultra: Fact or fiction?

Southern African journal of infectious diseases , 2024 | Pubmed ID: 38628427

Isolation of pure non-tuberculous mycobacteria culture from a mixed growth with conventional microbiology: Techniques not to be forgotten.

The Indian journal of tuberculosis Jul, 2024 | Pubmed ID: 39111929

Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study.

Diagnostic microbiology and infectious disease Dec, 2024 | Pubmed ID: 39293319

Targeted deep sequencing of mycobacteria species from extrapulmonary sites not identified by routine line probe assays: A retrospective laboratory analysis of stored clinical cultures.

IJID regions Dec, 2024 | Pubmed ID: 39483154

Mixed nontuberculous mycobacteria in an immunocompromised patient with probable progressive multifocal leukoencephalopathy.

IJID regions Mar, 2025 | Pubmed ID: 39810748

Xpert MTB/RIF Ultra-resistant and MTBDRsusceptible rifampicin results in people with tuberculosis: utility of FluoroType MTBDR and deep sequencing.

Antimicrobial agents and chemotherapy Mar, 2025 | Pubmed ID: 39918315

Waste to Worth: A diagnostic accuracy of Xpert MTB/XDR on contaminated liquid cultures to salvage the detection of drug-resistant tuberculosis.

Research square Apr, 2025 | Pubmed ID: 40297690

DNA extraction: Implications for NTM identification and amplicon sequencing.

Southern African journal of infectious diseases , 2025 | Pubmed ID: 40469625

INSTITUTIONS

Stellenbosch University

Green Point Tuberculosis Laboratory

JOVE ARTICLES

Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples

Jennifer Williams^*,1,

Janré Steyn^*,1,

Emilyn Costa Conceição^*,1,

Felicia Bernita Wells¹,

Melanie Grobbelaar¹,

Nabila Ismail¹,

Yonas Ghebrekristos^1,2,

Christoffel Johannes Opperman^1,2,3,4,

Sarishna Singh^1,2,

Jason Limberis⁵,

Fahd Naufal⁵,

Brendon Coenrad Mann¹,

Rebecca E. Colman^6,7,

Timothy Rodwell^*,6,7,

Robin Mark Warren^*,1,

John Z. Metcalfe^*,5

¹Division of Molecular Biology and Human Genetics, SAMRC Centre for TB Research, DSI-NRF Centre of Excellence for Biomedical TB Research, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University,

²National Health Laboratory Service, Green Point Tuberculosis Laboratory,

³Division of Medical Microbiology, Department of Pathology, University of Cape Town,

⁴Division of Medical Microbiology, Department of Pathology, University of Cape Town,

⁵Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco,

⁶Department of Medicine, University of California, San Diego, School of Medicine,

⁷Foundation for Innovative New Diagnostics (FIND), Campus Biotech

Genetics

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