Visualization and Mapping of Methoxy-X04-Labeled Amyloid Plaques in an Alzheimer’s Disease Mouse Brain Section

Begin with a mouse brain tissue section containing the region of interest in a buffer to maintain hydration during imaging.

The tissue is obtained from an Alzheimer's disease mouse model injected with methoxy X04, a fluorescent dye.

Methoxy X04 specifically binds to β-pleated sheet structures in amyloid plaques and retains its fluorescence for imaging and analysis.

Using a fluorescent microscope with a UV filter, excite the methoxy-X04 and capture the emitted fluorescence to visualize fluorescently labeled amyloid plaques against the surrounding tissue.

Without adjusting the microscope stage, capture images of the same region in bright-field mode to detail tissue structure and fluorescence mode to visualize the plaques.

Align the bright-field and fluorescent images using image processing software to precisely map the plaques onto the anatomical structures.

These images provide detailed visualization of amyloid plaques within their structural context to study Alzheimer's disease pathology.