Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
2.7K Views
•
08:57 min
•
November 11th, 2022
DOI :
November 11th, 2022
•Serena L. Cornelius1,2, Megan M. Colonnetta2,3,4,5, Katherine E. Lake1,2, Clayton A. Smith1,2, Yu-An Zhang1,2, Evanthia T. Roussos-Torres6, Sangeetha M. Reddy1,2, Elizabeth H. Chen2,3,4,5, Isaac S. Chan1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California
Chapters in this video
0:04
Introduction
0:55
Collecting, Digesting Tissue, and Differential Centrifugation
4:11
Embedding Organoids in BECM and Collagen
7:22
Results: Immunofluorescent Imaging of Embedded Organoids
8:11
Conclusion
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