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CAM-Delam Assay to Score Metastatic Properties by Quantifying Delamination and Invasion Capacity of Cancer Cells

2.6K Views

12:14 min

June 2nd, 2022

DOI :

10.3791/64025-v

June 2nd, 2022


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CAM Delam Assay

Chapters in this video

0:05

Introduction

1:00

Egg Incubation, Preparation of Weighing Boat, Plastic Boxes, and Dissection Instruments

1:46

Opening the Eggs, Transferring to the Internal Humidified Chamber, and Preparing Silicone Rings

3:07

Preparation and Seeding the Cancer Cells on the CAM

5:31

Isolation of the CAM with Associated Cancer Cells and Sectioning CAM-Delam Samples

7:28

Immunohistochemistry Staining, Microscopy Imaging, and Delamination Scoring

9:40

Results: CAM-Delam Assay to Evaluate the Metastatic Capacity of Cancer Cells

11:33

Conclusion

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