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A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

5.9K Views

07:03 min

June 16th, 2022

DOI :

10.3791/64016-v

June 16th, 2022


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Osteoclastic Resorption

Chapters in this video

0:04

Introduction

0:51

Calcification of 96-Well Cell Culture Plates

1:54

Isolation of Peripheral Blood Mononuclear Cells (PBMCs)

2:49

Expansion of Osteoclasts Progenitors and Induction of Osteoclastogenesis

4:19

Fluorescence Staining of Osteoclasts and Calcium Phosphate Coating

5:17

Results: Analysis of Mature Osteoclast Cultured on Calcium Phosphate Coated Wells

6:38

Conclusion

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