Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
3.8K Views
•
12:19 min
•
April 22nd, 2022
DOI :
April 22nd, 2022
•Hui-Lan Chang*1, Kang-Yi Su*1,2, Steven D. Goodman3, Wern-Cherng Cheng2, Liang-In Lin1,2, Ya-Chien Yang1,2, Sui-Yuan Chang1,2, Woei-horng Fang1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University
Chapters in this video
0:04
Introduction
1:28
DNA Glycosylase Assay
4:43
Transferring UDG Reaction Products to a Matrix Chip
5:33
Setting up the Assay Parameters on the Mass Spectrometer
7:19
Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data
9:03
Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis
11:01
Conclusion
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