JoVE Logo

Sign In

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

3.8K Views

12:19 min

April 22nd, 2022

DOI :

10.3791/63089-v

April 22nd, 2022


Explore More Videos

Uracil DNA Glycosylase

Chapters in this video

0:04

Introduction

1:28

DNA Glycosylase Assay

4:43

Transferring UDG Reaction Products to a Matrix Chip

5:33

Setting up the Assay Parameters on the Mass Spectrometer

7:19

Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data

9:03

Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis

11:01

Conclusion

Related Videos

article

07:49

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa

80.5K Views

article

09:53

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

12.9K Views

article

10:33

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

8.1K Views

article

11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

9.5K Views

article

11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

18.9K Views

article

06:56

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

25.0K Views

article

09:20

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

3.2K Views

article

12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

8.6K Views

article

09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

14.1K Views

article

Measuring In Vivo Adipose Tissue Kinetics in Humans Using the Deuterium (2H)-Labeling Approach

288 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved