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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

4.2K Views

10:08 min

May 21st, 2021

DOI :

10.3791/62075-v

May 21st, 2021


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High throughput Protein Purification

Chapters in this video

0:00

Introduction

0:58

Ni-NTA Chromatography- Purification of 24 Different Proteins: Preparing the Purification Set-up

3:00

Ni-NTA Chromatography- Purification of 24 Different Proteins: Loading, Washing and Eluting

4:47

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Preparing the Purification Set-up

6:15

Ion Exchange- Simultaneous Purification of 6 Different Proteins: Loading, Washing and Eluting

8:25

Results: Representative UV Spectroscopy and SDS PAGE Analyses

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