Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
4.4K Views
•
11:49 min
•
August 28th, 2021
DOI :
August 28th, 2021
•Chapters in this video
0:05
Introduction
0:49
Protein Extraction from Brain Tissue
2:04
Protein Quantification and Quality Check
2:32
Protein Digestion
3:58
Desalting and Peptide Quantification
5:06
Transition List Preparation of Finalised Targets
7:30
LC Parameters
8:03
MS Parameters
8:25
Run Sequence and Instrument QC
9:08
Method Refinement
10:58
Results and Conclusions
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