Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
12.0K Views
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06:22 min
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August 25th, 2020
DOI :
August 25th, 2020
•Ashwin Narayanan1, Enrique Blanco-Carmona2, Engin Demirdizen1, Xueyuan Sun1,3, Christel Herold-Mende4, Matthias Schlesner2, Sevin Turcan1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany
Chapters in this video
0:04
Introduction
1:01
Tissue Dissection and Dissociation
3:20
Gradient Centrifugation and Isolation of Nuclei
4:56
Results: Comparison of UMIs and Genes Obtained from Different Human Brain snRNA-seq Datasets
5:57
Conclusion
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