A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
5.5K Views
•
09:50 min
•
June 17th, 2020
DOI :
June 17th, 2020
•Hussein Atta*1,2, Ali Hashemi Gheinani*1,2, Amanda Wacker1, Yaser Heshmati3,4,5, Alex Bigger-Allen1,6, George Lambrinos1,2, Yao Gao2,7, Diane R. Bielenberg2,7, Rosalyn M. Adam1,2
1Department of Urology, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School, 3Division of Hematology/Oncology, Harvard Medical School Boston, 4Dana-Farber Cancer Institute, 5Broad Institute, 6Biological Biomedical Sciences Program, Division of Medical Sciences, Harvard Medical School, 7Vascular Biology Program, Boston Children's Hospital
Chapters in this video
0:04
Introduction
0:57
Mouse Preparation
1:48
Spinal Cord Injury (SCI)
3:17
Tissue Perfusion and Procurement
4:34
Cell Dissociation and Single Cell Suspension Preparation
5:59
Cell Staining
6:44
Flow Cytometry Analysis
7:43
Results: Representative Mouse Bladder Collagen Expression and Immune Cell Population Analyses
8:54
Conclusion
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