The authors like to thank Fynn Knudsen, Aravinda Bhari, and Jacob Muchynski for helpful discussions and the&#160;inspiration.

1. Printing of molds
<ol>
	<li>Download the *.stl data for molds from the Supplementary Data 1 &#8220;CAD/Moulds/&#8221;.</li>
	<li>Use the printer-specific slicing software to convert the 3D models into a print job.</li>
	<li>Print the molds using a 3D printer.</li>
	<li>Clean the printed molds by putting them for 15 min in an ultrasonic bath.</li>
	<li>Put the molds for at least 3 h in a UV chamber.</li>
</ol>
2. Preparing the elastomer
<ol>
	<li>Gather the following before starting this step: elastomer (part A and part B), spatula, plastic cup, mold, weight scale, plastic syringe, screw clamps (or similar), acrylic glass plate with two corresponding holes, cutter knife.</li>
	<li>Mix part A and part B of the elastomer in a 1:9 ratio in a cup. Place the cup on a weighing machine. First, add 5 g of part B (dark red). Then, using a spatula, add 45 g of part A (white and viscous). 
	NOTE: Ensure the accuracy of weighing is to 1 g. 50 g is enough for one actuator. The best way for portioning part A is to take a spatula and let it drain. Approximately 6 g per draining operation is possible with the spatula used.</li>
	<li>Keep stirring until no more white or red areas are visible at the edge of the cup.</li>
	<li>Put the cup for 15 min in a vacuum chamber to remove the air that is trapped in the elastomer due to the stirring process.</li>
	<li>Fill the mixed elastomer into a plastic syringe. This allows the elastomer to be positioned much more precisely. 
	NOTE: Supplementary Figure 1 illustrates the processing steps described in this section.</li>
</ol>
3. Manufacturing of upper part (base part)
<ol>
	<li>Clamp an acrylic glass plate with two corresponding holes onto the mold. Insert the syringe into the lower hole and press the elastomer into the mold.</li>
	<li>Apply force to the syringe by pushing the plunger until the mixed elastomer emerges from the upper hole.</li>
	<li>Loosen the screw clamps and pull off the acrylic glass plate sideways. 
	NOTE: It is important to pull it off to the side and not upwards. Otherwise, the elastomer will be pulled out of the mold.</li>
	<li>Puncture the rising air bubbles with a sharp tool. Do not puncture too deeply as this will create new air bubbles rather than remove the existing ones. It is especially important to pierce the larger bubbles as these will later significantly affect the functionality of the actuator. 
	NOTE: Optionally, evacuate the filled mold in the vacuum chamber to remove any air still trapped. When doing so, however, it can happen that the rising air bubbles get stuck on the mold on their way to the surface and create holes in the casting at functionally relevant areas. Supplementary Figure 2 illustrates this phenomenon.</li>
	<li>Put the mold into the oven at 65 &#176;C for 30 min.</li>
	<li>Check after 10 min if the level of the elastomer has fallen significantly. This happens if the mold is not completely tight or has bent slightly due to frequent use. If the level has dropped more than 1 mm, refill the elastomer. Then, continue curing.</li>
	<li>After a total of 30 min in the oven, take out the mold and cut off the extruded elastomer with a cutter knife.</li>
	<li>Open the mold by levering apart with a screwdriver. Be careful not to damage surfaces relevant for casting.</li>
	<li>Remove the almost finished actuator from the part of the mold to which it had stuck in the previous step. 
	NOTE: A first visual check can be made here to see if casting was successful. If irreparable defects are found (see Supplementary Figure 3), the manufacturing process is to be stopped here. Smaller holes can be repaired later. It is also important that the sealing lip is as pronounced as possible over its entire circumference.</li>
	<li>Cut off any protruding burrs with a cutter knife. This is sometimes very laborious, but essential for a good final result. 
	NOTE: Supplementary Figure 4 illustrates the processing steps described in this section. The described steps are valid for casting the four legs (the mold can be found in Supplementary File 1 &#8220;CAD/Moulds/small_leg_schwalbe*.stl&#8221;) and the two base parts of the torso (&#8220;CAD/Moulds/small_belly*.stl&#8221;). To cast the suction cups (feet of the robot, to be found in &#8220;CAD/Moulds/suctionCup*.stl&#8221;) or the bottom part of the torso (&#8220;CAD/Moulds/small_torso_base1*.stl&#8221;), carry out the same process steps, with the exception of steps 3.1 and 3.3 as these molds for casting have a built-in port for the syringe and therefore no additional acrylic glass plate is required. In total, build four base parts of the leg, two base parts of the torso, one bottom part of the torso, and four suction cups.</li>
</ol>
4. Manufacturing of lower part (bottom part)
<ol>
	<li>Push a silicone tube through the holes provided for this purpose in the mold of the bottom part, see Supplementary Figure 5.</li>
	<li>Fill the mold of the base part with elastomer and distribute it with the small spatula up to the corners. 
	NOTE: The level of the elastomer should be no higher than 5 mm and no lower than 4 mm and must completely cover the embedded tube. The mold for the bottom part of the legs can be found in Supplementary File 1 &#8220;CAD/Moulds/small_base_schwalbe.stl&#8221;.</li>
	<li>Put the mold into the oven for 15&#8211;20 min for curing. For the following steps, it is necessary that the bottom part remains in the mold for the time being joined with the top part.</li>
</ol>
5. Joining the base and bottom part
<ol>
	<li>Fill the mold of the bottom part with elastomer so that the level is 1&#8211;1.5 mm above the already hardened elastomer.</li>
	<li>Insert a butterfly cannula into the base part and mark the puncture site so that it can be found more easily later. This step is necessary to allow the expanding air in the oven to escape.</li>
	<li>Place the base part into the bottom mold and press only the sides slightly into the elastomer bath.</li>
	<li>Put the actuator into the oven for 10&#8211;15 min and remove the mold afterwards. 
	NOTE: It should be easy to remove the actuator from the mold. If it fails to do so, either the elastomer is not yet fully cured (in this case, increase the curing time by 10 more min) or the bottom part is stuck in the mold (in this case, it should be pulled harder). But in general, it is a bad sign if the actuator cannot be released easily.</li>
	<li>Connect a pressure source by using the puncture site from step 5.2 and perform the final leakage test, see Supplementary Figure 6. 
	NOTE: If small leaks are present, they can be repaired. Application of a little elastomer with a small spatula and 10 min in the oven should fix the leak. If all leaks are fixed, the actuator is ready. Supplementary Figure 6 illustrates the processing steps described in this section and Supplementary Figure 7 illustrates the entire process described in Sections 3&#8211;5. For joining the base and bottom part of the torso, perform the same steps, with exception of step 5.1, where you do not fill the mold but the bottom part directly.</li>
</ol>
6. Joining of all limbs
<ol>
	<li>Fix the parts to be joined with a pin needle on a wooden board so that they can be held together in the following process step.</li>
	<li>Cover the joining surface with elastomer as shown in Supplementary Figure 8A. Make sure that the joining surface is clean and free of fat. Otherwise, the parts will delaminate at this point.</li>
	<li>Put the assembly (see Supplementary Figure 8B) for 10&#8211;15 min into the oven.</li>
</ol>
7. Mounting of supply tube inlets
<ol>
	<li>Widen the insertion point of the butterfly cannula from step 5.2 further by using a 1 mm Allen key.</li>
	<li>Place the end of a silicone tube with a maximum outer diameter of 3 mm over the hole and press it in with the Allen key.</li>
	<li>Seal the inlet with a little elastomer. This also protects against mechanical stress.</li>
	<li>Put the assembly for 10 min into the oven. 
	NOTE: Supplementary Figure 9 illustrates the processing steps described in this section.</li>
</ol>
8. Building the control box
<ol>
	<li>Download the corresponding *.dxf drawings of the housing from Supplementary Data 1 &#8220;CAD/ControlBox/&#8221; and cut them out on a laser cutter.</li>
	<li>Assemble the &#8220;User Interface Unit&#8221; on the front panel according to Supplementary Figure 10A and Supplementary Figure 11.</li>
	<li>Build the six &#8220;Valve Units&#8221; according to Supplementary Figure 10B and Supplementary Figure 12.</li>
	<li>Assemble the six &#8220;Valve Units&#8221; and the &#8220;User Interface Unit&#8221; on the bottom panel according to Supplementary Figure 10C, Supplementary Figure 13, and Supplementary Figure 14. Assemble the two side panels and the back panel. Last, assemble the top panel.</li>
	<li>Configure the two single-board computers embedded in the control box according to Supplementary File 1 and upload the complete folder &#8220;Code&#8221; (including all subfolders) provided in the Supplementary Data 2 onto both boards.</li>
	<li>Upload the script &#8220;Code/arduino_p_ctr.ino&#8221; provided in the Supplementary Data 2 onto the six micro-controllers embedded in the control box.</li>
</ol>
9. Building a test bench with embedded measurement system
<ol>
	<li>Download the corresponding *.dxf drawing of the camera holder from Supplementary Data 1 &#8220;CAD/TestBench/&#8221; and cut it out on a laser cutter.</li>
	<li>Download the corresponding *.stl files of the clamps from Supplementary Data 1 &#8220;CAD/TestBench&#8221; and print them on a 3D-printer.</li>
	<li>Assemble the camera holder with the clamps on a DIN-A1 poster panel according to Supplementary Figure 15 and mount the camera and a single-board computer at the intended location.</li>
	<li>Configure the ethernet interface and the SSH settings of the single-board computer according to Sections 4&#8211;5 of Supplementary File 1 and upload the complete folder &#8220;Code&#8221; (Supplementary Data 2) onto the board.</li>
</ol>
10. Setting up the entire system
<ol>
	<li>Create a local network and assign the correct IP address from the script &#34;Code/main.py&#34; to all single-board computers and the computer used for monitoring &#8211; or rewrite the script accordingly.</li>
	<li>Insert pin needles into both ends of the torso as shown in Supplementary Figure 16, so that the robot only contacts the walking plane with the pins and its feet (suction cups).</li>
	<li>Print out the visual markers15 provided in Supplementary File 2 on a DIN-A4 sheet and cut them out using a scissor.</li>
	<li>Attach the markers to the robot using pin needles according to Supplementary Figure 17.</li>
	<li>Connect the robot to the control box. 
	NOTE: Figure 1 illustrates the wiring of the entire system.</li>
</ol>
11. Running the control box
<ol>
	<li>Power on the main switch of the control box and wait until everything is booted.</li>
	<li>Log into the main single-board computer as &#8220;root&#8221; using SSH, browse to the folder &#8220;Code&#8221;, and start the control box by the command &#8220;root@beaglebone:~# python3 main.py&#8221;. At the same time, start the monitor on the personal computer by the command &#8220;user@pc:~ python2 monitor.py&#8221;. 
	NOTE: Both programs must start more or less at the same time. The program &#8220;main.py&#8221; running on the single-board computer in the control box tries to connect to the personal computer used for monitoring. If there is no listening port at the personal computer (triggered by the script &#8220;monitor.py&#8221;), the monitor will not start. Except of &#8220;monitor.py&#8221;, all programs/scripts used in this protocol are intended to run with python3.</li>
	<li>Connect a pressure source to the control box (max. 1.2 bar).</li>
	<li>Connect a vacuum source to the control box.</li>
</ol>
12. Calibrating the robot
<ol>
	<li>Place the robot on the walking plane of the test bench. For steep inclines, attach a string between the robot&#8217;s front and the top of the walking plane in order to hold the robot in place.</li>
	<li>On the control box, activate the &#8220;pattern reference&#8221; mode by pushing the &#8220;mode 2&#8221; button as shown in Supplementary Figure 18.</li>
	<li>Scroll through the menu displayed on the LCD by using the up and down buttons until you find the entry &#8220;clb&#8221;. Then, push the enter button.</li>
	<li>Scroll through the next menu up to the entry &#8220;mode_4.csv&#8221; and push the &#34;enter&#34; button.</li>
	<li>On the monitor, press the &#8220;record&#8221; button as shown in the Supplementary Figure 19. 
	NOTE: Pressing the &#8220;record&#8221; button will automatically create a *.csv file on the monitoring computer at the location specified in &#8220;Code/Src/GUI/save.py:save_last_sample_as_csv()&#8221;, which is the folder &#8220;current_exp&#8221; (example measurements are provided in Supplementary Data 3).</li>
	<li>On the control box, press the &#8220;function 1&#8221; button to start the calibration procedure.</li>
	<li>After calibration, press the &#8220;record&#8221; button on the monitor to stop recording and the &#8220;function 1&#8221; button on the control box to stop the pressure controller.</li>
	<li>Rename the automatically created &#8220;current_exp/*.csv&#8221; file so that it can be uniquely identified later.</li>
	<li>Run the script &#8220;Calibration/eval_clb.py&#8221; provided in the Supplementary Data 4 and store the output (coefficients of the polynomial fit) in the file &#8220;Code/Src/Controller/calibration.py&#8221; as an entry with the keyword &#8220;[robot version]&#8221; within the existing dictionary.</li>
</ol>
13. Creating a gait pattern
<ol>
	<li>Run the script &#8220;Code/Patterns/create_pattern.py&#8221; and store the outputted *.csv file(s) in the folder &#8220;Code/Patterns/[robot version]/&#8221;. 
	NOTE: This script converts the predefined gait pattern for straight gait8 (see Supplementary Figure 20A or Supplementary Animation 1) formulated in angle references into robot-specific pressure references. To generate a gait pattern for steep inclines, modify the script by uncommenting line 222. This will generate a pattern according to Supplementary Figure 20B or Supplementary Animation 2. The interface for pattern references provided by the control box consists of *.csv files where each row defines a discrete set point for all actuators. Therein, the first eight columns define the reference pressures, the next four columns define the references for the direct acting valves, and the last column defines the time this set point should be held.</li>
	<li>Synchronize the single-board computer in the control box with the personal computer, i.e., upload the folder &#8220;Code/Pattern/*&#8221; onto the board. For this purpose, the program &#8220;main.py&#8221; has to be interrupted (Ctrl+C).</li>
</ol>
14. Carrying out the climbing experiment
<ol>
	<li>Perform steps 11&#8211;13 for each inclination to be tested.</li>
	<li>Place the robot at the marked point on the walking plane.</li>
	<li>Select a pattern reference as described in steps 12.2&#8211;12.4, but select in the first menu the desired &#8220;robot version&#8221; (instead of &#8220;clb&#8221;), and in the second menu the pattern reference according to the current inclination (instead of &#8220;mode_4.csv&#8221;).</li>
	<li>Start recording as described in step 12.5.</li>
	<li>Push the &#8220;function 1&#8221; button to activate the pressure controller.</li>
	<li>Let the robot walk/climb for at least 6 cycles.</li>
	<li>Stop recording by pushing the &#8220;record&#8221; button on the monitor (like in step 12.7).</li>
	<li>Make sure the robot will not fall when executing the next step.</li>
	<li>Stop the pressure controller by pushing again the &#8220;function 1&#8221; button. This will also stop the vacuum supply, and consequently the robot will fall.</li>
	<li>Move the recorded *.csv file into the folder &#8220;ExpEvaluation/[robot version]/[pattern type]/[inclination]/&#8221;. 
	NOTE: Repeat each run at least five times in order to have a solid base for the next step.</li>
</ol>
15. Evaluating the experiment
<ol>
	<li>Run the script &#8220;ExpEvaluation/eval_vS11_adj_ptrn.py&#8221; provided in Supplementary Data 5 to automatically mean over all measurement data. 
	NOTE: This script outputs the track of all feet, the applied pressure over time, the measured bending angle of all limbs over time, the velocity of the robot over time, the orientation of the robot over time, the mean velocity over inclination (cf. Figure 2A), and an approximation of the energy used over inclination (cf. Figure 2B).</li>
</ol>

<ol>
	<li>Majidi, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soft+robotics:+a+perspective-current+trends+and+prospects+for+the+future.">Soft robotics: a perspective-current trends and prospects for the future.</a> Soft Robotics. 1 (1), 5-11 (2014).</li><li>Kim, S., Laschi, C., Trimmer, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soft+robotics:+a+bioinspired+evolution+in+robotics.">Soft robotics: a bioinspired evolution in robotics.</a> Trends in Biotechnology. 31 (5), 287-294 (2013).</li><li>Rus, D., Tolley, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Design,+fabrication+and+control+of+soft+robots.">Design, fabrication and control of soft robots.</a> Nature. 521 (7553), 467-475 (2015).</li><li>Calisti, M., Picardi, G., Laschi, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fundamentals+of+soft+robot+locomotion.">Fundamentals of soft robot locomotion.</a> Journal of the Royal Society Interface. 14 (130), 0101 (2017).</li><li>Chu, B., Jung, K., Han, C. S., Hong, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+survey+of+climbing+robots:+locomotion+and+adhesion.">A survey of climbing robots: locomotion and adhesion.</a> International Journal of Precision Engineering and Manufacturing. 11 (4), 633-647 (2010).</li><li>Gu, G., Zou, J., Zhao, R., Zhao, X., Zhu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soft+wall-climbing+robots.">Soft wall-climbing robots.</a> Science Robotics. 3 (25), 2874 (2018).</li><li>Qin, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+versatile+soft+crawling+robot+with+rapid+locomotion.">A versatile soft crawling robot with rapid locomotion.</a> Soft Robotics. 6 (4), 455-467 (2019).</li><li>Seibel, A., Schiller, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+engineering+design+helps+creating+new+soft+machines.">Systematic engineering design helps creating new soft machines.</a> Robotics and Biomimetics. 5 (1), 5 (2018).</li><li>Schiller, L., Seibel, A., Schlattmann, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toward+a+gecko-inspired,+climbing+soft+robot.">Toward a gecko-inspired, climbing soft robot.</a> Frontiers in Neurorobotics. 13 (1), 106 (2019).</li><li>Mosadegh, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pneumatic+networks+for+soft+robotics+that+actuate+rapidly.">Pneumatic networks for soft robotics that actuate rapidly.</a> Advanced Functional Materials. 24 (15), 2163-2170 (2014).</li><li>Elango, N., Faudzi, A. A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+article:+investigations+on+soft+materials+for+soft+robot+manipulations.">A review article: investigations on soft materials for soft robot manipulations.</a> The International Journal of Advanced Manufacturing Technology. 80 (5-8), 1027-1037 (2015).</li><li>Natarajan, E., Razif, M. R., Faudzi, A., Palanikumar, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+a+suitable+material+for+soft+actuator+through+experiments+and+FE+simulations.">Evaluation of a suitable material for soft actuator through experiments and FE simulations.</a> International Journal of Manufacturing, Materials, and Mechanical Engineering. 10 (2), 64-76 (2020).</li><li>. Soft Robotics Toolkit [software] Available from: <a target="_blank" href="https://softroboticstoolkit.com">https://softroboticstoolkit.com</a> (2020)</li><li>. PneumaticBox [software] Available from: <a target="_blank" href="https://www.robotics.tu-berlin.de/menue/software_and_tutorials/pneumaticbox/">https://www.robotics.tu-berlin.de/menue/software_and_tutorials/pneumaticbox/</a> (2020)</li><li>Wang, J., Olson, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apriltag+2:+efficient+and+robust+fiducial+detection.">Apriltag 2: efficient and robust fiducial detection.</a> Proceedings of the IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS). , 4193-4198 (2016).</li></ol>

The authors declare that they have no competing financial interests.

The presented protocol includes many different aspects related to the climbing soft robot from Ref.9, including manufacturing, control, calibration, and performance evaluation. In the following, the pros and cons resulting from the protocol are discussed and structured according to the aspects mentioned above.
The presented manufacturing method is strongly based on the existing literature10,13. A substantial difference is the design of the actuator. To join the individual limbs, dovetail guides are inserted at&#160;appropriate points, as shown in Supplementary Figure 21. This results in a much more precise and robust connection between the limbs compared to the previous design&#160;of the robot8. Furthermore, the supply tubes are embedded in the bottom part of the actuators. This integrated design allows the suction cups to be supplied with vacuum and at the same time makes the bottom layer no longer stretchable, which significantly increases the performance of the actuator. Another difference to the procedure described in the literature is that the mixed elastomer is evacuated only once (immediately after mixing). Many sources recommend evacuating the elastomer twice: once after mixing and once after it has been filled into the mold. It may happen that air remains trapped in very small spaces. In the vacuum chamber, this air expands and in the best case rises to the surface. Often enough, however, these air bubbles get stuck on their way, creating unpleasant holes in the finished casting. Here, a decision must be made as to what is more important: perfect contours on the bottom side of the base part or as little risk as possible of producing a non-functional actuator&#160;(cf.&#160;Supplementary Figure 2). In this protocol, no second evacuation is performed. In the procedure presented, the height of the bottom part may vary as it is filled manually, and, unlike for the base part, there is no possibility of cutting it to a uniform height after curing. To ensure that the height of the bottom part is as uniform as possible, it is recommended to use a syringe when filling the mold of the bottom part and to measure the volume poured in. However, depending on how much time has elapsed since mixing, the flow properties of the elastomer change significantly. Therefore, it is recommended to always use freshly mixed elastomer. Joining the base and the bottom part of the actuator involves the largest process uncertainty. If the elastomer bath is too high, the air channel between the chambers will most likely be covered as well. Then, the actuator is no longer usable. If the elastomer bath is too low, the sealing lip may not be covered in its entire circumference and the actuator would leak. Therefore, it takes a certain amount of practice to dose the elastomer bath correctly. Important for joining in general is a fat-free joining surface. If the joining surface is too contaminated, the finished actuator may delaminate. Therefore, it is essential to ensure that the parts are only touched on surfaces that are not to be joined. A major limitation of the manufacturing method is the number of pieces to be realized. The production of a single actuator takes at least two hours in total. Although it is possible to work with several molds in parallel, more than four is not recommendable due to time constraints. The pot life of the elastomer is too short to be able to fill even more molds. In addition, the 3D-printed molds only withstand a limited number of production cycles (approx. 10&#8211;20) before they become very deformed or break. A further limitation is the process uncertainties already discussed. Since almost all process steps are performed manually, each actuator is a little different. This can lead to two robots that are identical in construction but show two very different behaviors.
With the control box, a method is provided to control the robot. Nevertheless, for each pneumatic system, the control gains of the script &#34;Code/arduino_p_ctr.ino&#34; must be determined individually. This is not covered in the protocol. However, the &#34;pressure reference mode&#34; of the control box allows a playful handling of the robot, so that controller tuning can be made without writing several scripts. Another limitation of the control box is its cost&#160;as the material costs about 7000 US$ in total. The literature11 offers a building instruction for a control box that costs only about 900 US$ and with some upgrades could also be used to operate the robot.
Critical for the calibration of the individual actuators is the choice of the calibration procedure. Supplementary Figure 22 shows the qualitative course of the pressure references over time for four different procedures and Supplementary Figure 23 shows the resulting angle pressure curves. As can be seen in the latter, each method of calibration results in a different angle-pressure curve. This shows that the relationship between pressure and angle is highly dependent on the load acting on the actuator. Therefore, the calibration procedure must reflect the real load case as best as possible. Consequently, it is necessary to adapt the calibration procedure to the real operating conditions as far as possible. The best walking performance is obtained with calibration procedure 4. However, as can be seen in Figure 3B, the subsequent poses in the series are not completely symmetrical, which is an indicator for the potential of improvement in calibration.
Critical to the measuring system is the assembly of the visual markers15 in Section 10. Since they cannot be mounted directly at the desired points (because the tubes interfere), the measured points must be shifted artificially. Special care must be taken when determining this offset vector (in pixel coordinates of the camera); otherwise, the entire measurement will have significant systematic errors. It must also be ensured that the tags do not displace with time. If this happens,&#160;e.g., due to a downfall of the robot, the corresponding tag must be remounted in the exact same place. In any case, it should be checked regularly whether the measuring system still produces reliable output.
The limiting factor in the experiment is the fixation of the feet. In order to be able to climb even steeper inclinations, the fixation mechanism must&#160;be reconsidered. Currently, the robot is not able to actively push its feet against the walking plane, and for high inclines, the normal force caused by gravity is too small to bring the suction cups close enough to the walking plane to ensure reliable suction.
The presented manufacturing method can be transferred to any fluidic elastomer actuator and could, therefore, be interesting for future applications. The presented control box enables the control of any pneumatic system consisting of six individual actuators (expandable up to eight), including robotic platforms as they require fast sensory feedback. Therefore, it could be used as a universal platform for testing and control future robots. Finally, the presented calibration method can be,&#160;in principle, to any feed-forward controlled pneumatic system. In summary, all presented methods are universal within the discussed scope.

The interaction between humans and machines is becoming constantly closer. The increasing robot density in companies and households poses new challenges for the robot technology. Frequently, dangers are excluded by separation methods, but in many areas, especially in households, this is not a satisfactory solution. Soft robotics tackles this problem by using properties of soft materials and structures to develop new types of machines that behave like living organisms1, which is why soft robots are often inspired by biological models2. Most soft robots can be classified into two different types: mobile robots and robots designed for gripping and manipulation3. For soft mobile robots, typical locomotion principles are crawling, walking, running, jumping, flying, and swimming4. Another interesting field of application for soft robots is climbing &#8211; a combination of locomotion and adhesion5. Soft machines are very robust and cannot damage their surroundings due to their softness. This characteristic predestines this robot class for climbing, as they can easily survive a fall. Consequently, the literature offers several examples of soft robots capable of climbing6,7,8.
The goal of this protocol is to provide a method to manufacture, control, and evaluate the performance of a gecko-inspired, climbing soft robot9. Its design is based on the use of fast pneunet soft bending actuators10 made of elastomer. However, another soft actuator design and/or material could also be used. The literature offers a wide range of different designs of soft actuators11 and suitable materials12. The presented manufacturing method is similar to existing methods13 but includes some modifications that result in increased repeatability and robustness, at least in the case of the soft climbing robot9. The method is valid for fast pneunet bending actuators in general and might, therefore, be interesting for newcomers to the field of actuator manufacturing.
For controlling pneumatic actuated soft robots, the literature provides different solutions. It ranges from low-cost and easy-to-replicate control boards13 to powerful but more complex boards14, which cannot be rebuilt without special tools. Here, a brief description is provided for building a pneumatic control box by only using a laser cutter and a soldering iron. The control box allows the supply of any pressure and offers real-time sensory feedback, which is especially important for robotics applications. However, it can also be used for many other applications.

<table><tbody><tr><td>3D Printer</td><td>Formlabs</td><td>Form 2</td><td></td></tr><tr><td>acrylic glass plate with two holes</td><td>-</td><td></td><td>for casting, see Supplementary</td></tr><tr><td>acrylic glass back panel</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>acrylic glass bottom panel</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>acrylic glass front panel</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>acrylic glass side panel</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>acrylic glass top panel</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>Arduino Nano</td><td>Arduino</td><td>A000005</td><td></td></tr><tr><td>Allan Key 1mm</td><td></td><td></td><td>available in every workshop</td></tr><tr><td>BeagleBone Black</td><td>beagleboard</td><td>BBB01-SC-505</td><td></td></tr><tr><td>butterfly cannula</td><td>B. Braun Melsungen AG</td><td>5039573</td><td></td></tr><tr><td>clamp 1 for measurement system</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>Clamp 2 for measurement system</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>cutter knife</td><td></td><td></td><td>available in every workshop</td></tr><tr><td>direct acting solenoid valves</td><td>Norgren</td><td>EXCEL22 DM/49/MDZ83J/T4</td><td></td></tr><tr><td>elastomer</td><td>Wacker Chemie</td><td>ELASTOSIL M4601</td><td></td></tr><tr><td>frame measurement system part 1</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>frame measurement system part 2</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>laser cutter</td><td>Trotec</td><td>SP500</td><td></td></tr><tr><td>LED</td><td>RND COMPONENTS</td><td>RND 210-00013</td><td></td></tr><tr><td>LCD</td><td>JOY-IT</td><td>SBC-LCD16X2</td><td></td></tr><tr><td>mould bottom part leg</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould bottom part torso 1</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould bottom part torso 2</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould leg 1</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould leg 2</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould torso 1</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>mould torso 2</td><td>-</td><td></td><td>see Supplementary</td></tr><tr><td>oven</td><td>Binder</td><td>ED 115</td><td></td></tr><tr><td>Plastic Cup</td><td></td><td></td><td>available in every supermarket</td></tr><tr><td>Plastic syringe</td><td></td><td></td><td>available in every pharmacy</td></tr><tr><td>poster panel</td><td>Net-xpress.de (distributor)</td><td>10620232</td><td>as walking plane</td></tr><tr><td>Potentiometer</td><td>VISHAY</td><td>P16NM103MAB15</td><td></td></tr><tr><td>Power Supply</td><td>Pulse Dimension</td><td>CPS20.241-C1</td><td></td></tr><tr><td>pressure sensor</td><td>Honeywell</td><td>SSCDANN150PG2A5</td><td></td></tr><tr><td>Pressure Source</td><td>EINHELL</td><td>4020600</td><td></td></tr><tr><td>proportional valves</td><td>Festo</td><td>MPYE-5-1/8-LF-010-B</td><td>6x</td></tr><tr><td>Raspberry Pi</td><td>RASPBERRY PI</td><td>RASPBERRY PI 3B+</td><td></td></tr><tr><td>Raspberry Pi Cam</td><td>RASPBERRY PI</td><td>RASPBERRY PI CAMERA V2.1</td><td></td></tr><tr><td>resin</td><td>formlabs</td><td>grey resin 1l</td><td></td></tr><tr><td>screw clamps</td><td>VELLEMAN</td><td>3935-12</td><td></td></tr><tr><td>silicon tube 2mm</td><td>Festo</td><td>PUN-H-2X0,4-NT</td><td>for connecting robot to control box</td></tr><tr><td>silicone Tube 2.5mm</td><td>Schlauch24</td><td>n/a</td><td>for supply tube inlet (https://www.ebay.de/itm/281761715815)</td></tr><tr><td>Switches</td><td>MIYAMA</td><td>MS 165</td><td></td></tr><tr><td>ultrasonic bath</td><td>RND LAB</td><td>605-00034</td><td></td></tr><tr><td>UV chamber</td><td>formlabs</td><td>Form Cure</td><td></td></tr><tr><td>Vacuum chamber + pump</td><td>COPALTEC</td><td>PURE PERFEKTION</td><td></td></tr><tr><td>weight scale</td><td>KERN-SOHN</td><td>PCB 2500-2</td><td>min. resolution 1g</td></tr></tbody></table>

manufacturing, control, and performance evaluation of a gecko-inspired soft robot

This protocol presents a method for manufacturing, control, and evaluation of the performance of a soft robot that can climb inclined flat surfaces with slopes of up to 84&#176;. The manufacturing method is valid for the fast pneunet bending actuators in general and might, therefore, be interesting for newcomers to the field of actuator manufacturing. The control of the robot is achieved by means of a pneumatic control box that can provide arbitrary pressures and can be built by only using purchased components, a laser cutter, and a soldering iron. For the walking performance of the robot, the pressure-angle calibration plays a crucial role. Therefore, a semi-automated method for the pressure-angle calibration is presented. At high inclines (&#62; 70&#176;), the robot can no longer reliably fix itself to the walking plane. Therefore, the gait pattern is modified to ensure that the feet can be fixed on the walking plane.

Watch this Scientific Journal Video about Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot at JoVE.com

Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot

This protocol provides a detailed list of steps to be performed for the manufacturing, control and evaluation of the climbing performance of a gecko-inspired soft robot.

This protocol presents a method for manufacturing, control, and evaluation of the performance of a soft robot that can climb inclined flat surfaces with ...

manufacturing-control-performance-evaluation-gecko-inspired-soft

Universidad San Sebastian

Research

JoVE Journal

Engineering

13.8K Views.  Hamburg University of Technology. This protocol provides a detailed list of steps to be performed for the manufacturing, control and evaluation of the climbing performance of a gecko-inspired soft robot.

Video: Manufacturing, Control, and Performance Evaluation of a Gecko-Inspired Soft Robot

The Modular Design and Production of an Intelligent Robot Based on a Closed-Loop Control Strategy

Intelligent robots are part of a new generation of robots that are able to sense the surrounding environment, plan their own actions and eventually reach their targets. In recent years, reliance upon robots in both daily life and industry has increased. The protocol proposed in this paper describes the design and production of a handling robot with an intelligent search algorithm and an autonomous identification function.
First, the various working modules are mechanically assembled to complete the construction of the work platform and the installation of the robotic manipulator. Then, we design a closed-loop control system and a four-quadrant motor control strategy, with the aid of debugging software, as well as set steering gear identity (ID), baud rate and other working parameters to ensure that the robot achieves the desired dynamic performance and low energy consumption. Next, we debug the sensor to achieve multi-sensor fusion to accurately acquire environmental information. Finally, we implement the relevant algorithm, which can recognize the success of the robot&#39;s function for a given application.
The advantage of this approach is its reliability and flexibility, as the users can develop a variety of hardware construction programs and utilize the comprehensive debugger to implement an intelligent control strategy. This allows users to set personalized requirements based on their needs with high efficiency and robustness.

We present a protocol on modular design and production of intelligent robots to help scientific and technical workers design intelligent robots with special production tasks based on personal needs and individualized design.

Intelligent robots are part of a new generation of robots that are able to sense the surrounding environment, plan their own actions and eventually reach ...

Fabrication of Soft Pneumatic Network Actuators with Oblique Chambers

Soft pneumatic network actuators have become one of the most promising actuation devices in soft robotics which benefits from their large bending deformation and low input. However, their monotonous bending motion form in two-dimensional (2-D) space keeps them away from wide applications. This paper presents a detailed fabrication method of soft pneumatic network actuators with oblique chambers, to explore their motions in three-dimensional (3-D) space. The design of oblique chambers enables actuators with tunable coupled bending and twisting capabilities, which gives them the possibility to move dexterously in flexible manipulators, to become biologically inspired robots and medical devices. The fabrication process is based on the molding method, including the silicone elastomer preparation, chamber and base parts fabrication, actuator assembly, tubing connections, checks for leaks, and actuator repair. The fabrication method guarantees the rapid manufacturing of a series of actuators with only a few modifications in the molds. The test results show the high quality of the actuators and their prominent bending and twisting capabilities. Experiments of the gripper demonstrate the advantages of the development in adapting to objects with different diameters and providing sufficient friction.

Here we present a fabrication method of soft pneumatic network actuators with oblique chambers. The actuators are capable of generating coupled bending and twisting motions, which broadens their application in soft robotics.

Soft pneumatic network actuators have become one of the most promising actuation devices in soft robotics which benefits from their large bending ...

Design and Fabrication of an Elastomeric Unit for Soft Modular Robots in Minimally Invasive Surgery

In recent years, soft robotics technologies have aroused increasing interest in the medical field due to their intrinsically safe interaction in unstructured environments. At the same time, new procedures and techniques have been developed to reduce the invasiveness of surgical operations. Minimally Invasive Surgery (MIS) has been successfully employed for abdominal interventions, however standard MIS procedures are mainly based on rigid or semi-rigid tools that limit the dexterity of the clinician. This paper presents a soft and high dexterous manipulator for MIS. The manipulator was inspired by the biological capabilities of the octopus arm, and is designed with a modular approach. Each module presents the same functional characteristics, thus achieving high dexterity and versatility when more modules are integrated. The paper details the design, fabrication process and the materials necessary for the development of a single unit, which is fabricated by casting silicone inside specific molds. The result consists in an elastomeric cylinder including three flexible pneumatic actuators that enable elongation and omni-directional bending of the unit. An external braided sheath improves the motion of the module. In the center of each module a granular jamming-based mechanism varies the stiffness of the structure during the tasks. Tests demonstrate that the module is able to bend up to 120&#176; and to elongate up to 66% of the initial length. The module generates a maximum force of 47 N, and its stiffness can increase up to 36%.

This paper describes the design and fabrication of a soft unit for surgical manipulators. The base module includes three flexible fluidic actuators to achieve omnidirectional bending and elongation, and a granular jamming-based mechanism to enable stiffness control. A complete mechanical characterization is also reported.

In recent years, soft robotics technologies have aroused increasing interest in the medical field due to their intrinsically safe interaction in ...

Bioengineering

Rapid Manufacturing of Thin Soft Pneumatic Actuators and Robots

This protocol describes a method for rapid manufacturing of soft pneumatic actuators and robots with an ultrathin form factor using a heat press and a laser cutter machine. The method starts with the lamination of thermoplastic polyurethane (TPU) sheets using a heat press for 10 min at the temperature of ~93 &#176;C. Next, the parameters of the laser cutter machine are optimized to produce a rectangular balloon with maximum burst pressure. Using the optimized parameters, the soft actuators are laser cut/welded three times sequentially. Next, a dispensing needle is attached to the actuator, allowing it to be inflated. The effect of geometrical parameters on the deflection of the actuator are studied systematically by varying the channel width and length. Finally, the performance of the actuator is characterized using an optical camera and a fluid dispenser. Conventional fabrication methods of soft pneumatic actuators based on silicone molding are time consuming (several hours). They also result in strong but bulky actuators, which limits the actuator&#8217;s applications. Moreover, microfabrication of thin pneumatic actuators is both time-consuming and expensive. The proposed manufacturing method in the current work resolves these issues by introducing a fast, simple, and cost-effective fabrication method of ultrathin pneumatic actuators.

This protocol describes a method for rapid manufacturing of soft pneumatic actuators and robots with a thin form factor. The fabrication method starts with lamination of thermoplastic polyurethane (TPU) sheets followed by laser cutting/welding of a two-dimensional pattern to form actuators and robots.

This protocol describes a method for rapid manufacturing of soft pneumatic actuators and robots with an ultrathin form factor using a heat press and a ...

Rod-based Fabrication of Customizable Soft Robotic Pneumatic Gripper Devices for Delicate Tissue Manipulation

Soft compliant gripping is essential in delicate surgical manipulation for minimizing the risk of tissue grip damage caused by high stress concentrations at the point of contact. It can be achieved by complementing traditional rigid grippers with soft robotic pneumatic gripper devices. This manuscript describes a rod-based approach that combined both 3D-printing and a modified soft lithography technique to fabricate the soft pneumatic gripper. In brief, the pneumatic featureless mold with chamber component is 3D-printed and the rods were used to create the pneumatic channels that connect to the chamber. This protocol eliminates the risk of channels occluding during the sealing process and the need for external air source or related control circuit. The soft gripper consists of a chamber filled with air, and one or more gripper arms with a pneumatic channel in each arm connected to the chamber. The pneumatic channel is positioned close to the outer wall to create different stiffness in the gripper arm. Upon compression of the chamber which generates pressure on the pneumatic channel, the gripper arm will bend inward to form a close grip posture because the outer wall area is more compliant. The soft gripper can be inserted into a 3D-printed handling tool with two different control modes for chamber compression: manual gripper mode with a movable piston, and robotic gripper mode with a linear actuator. The double-arm gripper with two actuatable arms was able to pick up objects of sizes up to 2 mm and yet generate lower compressive forces as compared to elastomer-coated and non-coated rigid grippers. The feasibility of having other designs, such as single-arm or hook gripper, was also demonstrated, which further highlighted the customizability of the soft gripper device, and it's potential to be used in delicate surgical manipulation to reduce the risk of tissue grip damage.

This protocol describes a rod-based approach, combining 3D-printing and soft lithography techniques for fabricating the soft gripper devices. This approach eliminates the need for an external air source by incorporating a chamber component and reduces the chance of occlusion during the sealing process, particularly for miniaturized pneumatic channels.

Soft compliant gripping is essential in delicate surgical manipulation for minimizing the risk of tissue grip damage caused by high stress concentrations ...

Four-Dimensional Printing of Stimuli-Responsive Hydrogel-Based Soft Robots

The present protocol describes the creation of four-dimensional (4D), time-dependent, shape-changeable, stimuli-responsive soft robots using a three-dimensional (3D) bio-printing method. Recently, 4D printing techniques have been extensively proposed as innovative new methods for developing shape-transformable soft robots. In particular, 4D time-dependent shape transformation is an essential factor in soft robotics because it allows effective functions to occur at the right time and place when triggered by external cues, such as heat, pH, and light. In line with this perspective, stimuli-responsive materials, including hydrogels, polymers, and hybrids, can be printed to realize smart shape-transformable soft robotic systems. The current protocol can be used to fabricate thermally responsive soft grippers composed of N-isopropylacrylamide (NIPAM)-based hydrogels, with overall sizes ranging from millimeters to centimeters in length. It is expected that this study will provide new directions for realizing intelligent soft robotic systems for various applications in smart manipulators (e.g., grippers, actuators, and pick-and-place machines), healthcare systems (e.g., drug capsules, biopsy tools, and microsurgeries), and electronics (e.g., wearable sensors and fluidics).

This manuscript describes a 4D printing strategy for fabricating intelligent stimuli-responsive soft robots. This approach can provide the groundwork to facilitate the realization of intelligent shape-transformable soft robotic systems, including smart manipulators, electronics, and healthcare systems.

The present protocol describes the creation of four-dimensional (4D), time-dependent, shape-changeable, stimuli-responsive soft robots using a ...

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Biological machines often referred to as biorobots, are living cell- or tissue-based devices that are powered solely by the contractile activity of living components. Due to their inherent advantages, biorobots are gaining interest as alternatives to traditional fully artificial robots. Various studies have focused on harnessing the power of biological actuators, but only recently studies have quantitatively characterized the performance of biorobots and studied their geometry to enhance functionality and efficiency. Here, we demonstrate the development of a self-stabilizing swimming biorobot that can maintain its pitch, depth, and roll without external intervention. The design and fabrication of the PDMS scaffold for the biological actuator and biorobot followed by the functionalization with fibronectin is described in this first part. In the second part of this two-part article, we detail the incorporation of cardiomyocytes and characterize the biological actuator and biorobot function. Both incorporate a base and tail (cantilever) which produce fin-based propulsion. The tail is constructed with soft lithography techniques using PDMS and laser engraving. After incorporating the tail with the device base, it is functionalized with a cell adhesive protein and seeded confluently with cardiomyocytes. The base of the biological actuator consists of a solid PDMS block with a central glass bead (acts as a weight). The base of the biorobot consists of two composite PDMS materials, Ni-PDMS and microballoon-PDMS (MB-PDMS). The nickel powder (in Ni-PDMS) allows magnetic control of the biorobot during cells seeding and stability during locomotion. Microballoons (in MB-PDMS) decrease the density of MB-PDMS, and enable the biorobot to float and swim steadily. The use of these two materials with different mass densities, enabled precise control over the weight distribution to ensure a positive restoration force at any angle of the biorobot. This technique produces a magnetically controlled self-stabilizing swimming biorobot.

In this two-part study, a biological actuator was developed using highly flexible polydimethylsiloxane (PDMS) cantilevers and living muscle cells (cardiomyocytes), and characterized. The biological actuator was incorporated with a base made of modified PDMS materials to build a self-stabilizing, swimming biorobot.

Biological machines often referred to as biorobots, are living cell- or tissue-based devices that are powered solely by the contractile activity of living ...

Revolutionizing Stroke Rehabilitation with Enhanced Grasping and Finger Recovery

A Flexible Wearable Supernumerary Robotic Limb for Chronic Stroke Patients

In this study, we present a flexible wearable supernumerary robotic limb that helps chronic stroke patients with finger rehabilitation and grasping movements. The design of this innovative limb draws inspiration from bending pneumatic muscles and the unique characteristics of an elephant's trunk tip. It places a strong emphasis on crucial factors such as lightweight construction, safety, compliance, waterproofing, and achieving a high output-to-weight/pressure ratio. The proposed structure enables the robotic limb to perform both envelope and fingertip grasping. Human-robot interaction is facilitated through a flexible bending sensor, detecting the wearer's finger movements and connecting them to motion control via a threshold segmentation method. Additionally, the system is portable for versatile daily use. To validate the effectiveness of this innovation, real-world experiments involving six chronic stroke patients and three healthy volunteers were conducted. The feedback received through questionnaires indicates that the designed mechanism holds immense promise in assisting chronic stroke patients with their daily grasping activities, potentially improving their quality of life and rehabilitation outcomes.

Author Spotlight: Enhancing Grasping Abilities for Hemiplegic Patients with Flexible Robotic Limbs 

This protocol introduces a flexible wearable supernumerary robotic limb tailored to assist in finger rehabilitation for stroke patients. The design incorporates a bending sensor to facilitate seamless human-robot interaction. Validation through experiments involving both healthy volunteers and stroke patients underscores the efficacy and dependability of the proposed study.

In this study, we present a flexible wearable supernumerary robotic limb that helps chronic stroke patients with finger rehabilitation and grasping ...

Insect-machine Hybrid System: Remote Radio Control of a Freely Flying Beetle (Mercynorrhina torquata)

The rise of radio-enabled digital electronic devices has prompted the use of small wireless neuromuscular recorders and stimulators for studying in-flight insect behavior. This technology enables the development of an insect-machine hybrid system using a living insect platform described in this protocol. Moreover, this protocol presents the system configuration and free flight experimental procedures for evaluating the function of the flight muscles in an untethered insect. For demonstration, we targeted the third axillary sclerite (3Ax) muscle to control and achieve left or right turning of a flying beetle. A thin silver wire electrode was implanted on the 3Ax muscle on each side of the beetle. These were connected to the outputs of a wireless backpack (i.e., a neuromuscular electrical stimulator) mounted on the pronotum of the beetle. The muscle was stimulated in free flight by alternating the stimulation side (left or right) or varying the stimulation frequency. The beetle turned to the ipsilateral side when the muscle was stimulated and exhibited a graded response to an increasing frequency. The implantation process and volume calibration of the 3 dimensional motion capture camera system need to be carried out with care to avoid damaging the muscle and losing track of the marker, respectively. This method is highly beneficial to study insect flight, as it helps to reveal the functions of the flight muscle of interest in free flight.

Insect-machine Hybrid System: Remote Radio Control of a Freely Flying Beetle Mercynorrhina torquata

This protocol describes the process of constructing an insect-machine hybrid system and carrying out wireless electrical stimulation of the flight muscles required to control the turning motion of a flying insect.

The rise of radio-enabled digital electronic devices has prompted the use of small wireless neuromuscular recorders and stimulators for studying in-flight ...

Neuroscience

Insect-controlled Robot: A Mobile Robot Platform to Evaluate the Odor-tracking Capability of an Insect

Robotic odor source localization has been a challenging area and one to which biological knowledge has been expected to contribute, as finding odor sources is an essential task for organism survival. Insects are well-studied organisms with regard to odor tracking, and their behavioral strategies have been applied to mobile robots for evaluation. This "bottom-up" approach is a fundamental way to develop biomimetic robots; however, the biological analyses and the modeling of behavioral mechanisms are still ongoing. Therefore, it is still unknown how such a biological system actually works as the controller of a robotic platform. To answer this question, we have developed an insect-controlled robot in which a male adult silkmoth (Bombyx mori) drives a robot car in response to odor stimuli; this can be regarded as a prototype of a future insect-mimetic robot. In the cockpit of the robot, a tethered silkmoth walked on an air-supported ball and an optical sensor measured the ball rotations. These rotations were translated into the movement of the two-wheeled robot. The advantage of this "hybrid" approach is that experimenters can manipulate any parameter of the robot, which enables the evaluation of the odor-tracking capability of insects and provides useful suggestions for robotic odor-tracking. Furthermore, these manipulations are non-invasive ways to alter the sensory-motor relationship of a pilot insect and will be a useful technique for understanding adaptive behaviors.

The capability to localize an odor source is necessary for insect survival and is expected to be applicable to artificial odor-tracking. The insect-controlled robot is driven by an actual silkmoth and enables us to evaluate the odor-tracking capability of insects through a robotic platform.

Robotic odor source localization has been a challenging area and one to which biological knowledge has been expected to contribute, as finding odor ...