Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
5.8K Views
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10:01 min
•
April 8th, 2020
DOI :
April 8th, 2020
•Andreas Schreiber*1,2, Lara G. Stühn*1,2, Süreyya E. Geissinger1,2, Matthias C. Huber1,2, Stefan M. Schiller1,2,3,4,5,6
1Center for Biological Systems Analysis, University of Freiburg, 2Faculty of Biology, University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, 4BIOSS Centre for Biological Signalling Studies, University of Freiburg, 5IMTEK Department of Microsystems Engineering, University of Freiburg, 6Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg
Chapters in this video
0:05
Introduction
0:45
Protein Expression and Preparation
3:38
Dye-modification of Elastin-like Proteins via SPAAC
4:44
THF Swelling Protocol
6:29
BuOH Extrusion Protocol
7:19
Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
9:22
Conclusion
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