Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
6.9K Views
•
09:19 min
•
March 16th, 2020
DOI :
March 16th, 2020
•Chapters in this video
0:04
Introduction
0:45
Cell Membrane Solubilization and Protein Extraction
3:53
UV-VIS Spectroscopy
4:33
Automated Size-Exclusion Chromatography of Rhodopsin and Rhodopsin Mini-Go Complex
6:04
Deglycosylation
6:44
Results: UV-VIS Spectroscopy, SEC, and SDS-PAGE
8:34
Conclusion
Related Videos
11:20
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
16.3K Views
08:53
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
29.8K Views
08:21
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
6.8K Views
09:03
Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
12.0K Views
14:04
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
4.6K Views
06:29
Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
5.3K Views
08:35
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
4.9K Views
08:46
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
2.1K Views
06:19
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
4.0K Views
09:27
Protein Crystallization for X-ray Crystallography
63.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved