TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
23.8K Views
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07:02 min
•
May 17th, 2020
DOI :
May 17th, 2020
•Yongliang Zhang*1, Yuanyuan Li*2,3, Xinxin Yang1, Zhiyan Wen1, Ugrappa Nagalakshmi2, Savithramma P. Dinesh-Kumar2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
Chapters in this video
0:04
Introduction
1:01
Agroinfiltration and Leaf Sample Collection
1:59
Extraction of Leaf Total Protein and Removal of Free Biotin
4:11
Enrichment of Biotinylated Proteins
5:53
Results: Western Blot of Protein Extracts
6:25
Conclusion
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