Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice
10.8K Views
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10:04 min
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November 12th, 2019
DOI :
November 12th, 2019
•Ryosuke Fujiki1,2,3,4,9, Joun Y. Lee1,2,10, Julie A. Jurgens1,2,3,7, Mary C. Whitman2,5,6, Elizabeth C. Engle1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine
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Chapters in this video
0:05
Title
1:17
Ventral Midbrain and Spinal Cord Dissection
6:10
Tissue Dissociation
7:23
Culture of Purified Primary Motor Neurons
7:59
Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
9:34
Conclusion
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