Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
15.6K Views
•
09:48 min
•
September 26th, 2019
DOI :
September 26th, 2019
•1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine
Chapters in this video
0:04
Title
1:00
Harvesting the Colon
1:32
Cleaning the Colon
3:10
Collagenase Digestion 1
3:34
Silica-Based Density Separation Media Gradient Separation
5:44
Collect Mononuclear Cells from the Gradient Interface
6:39
Results: Isolation of Lamina Propria Mononuclear Cells
8:40
Conclusion
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