Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology
34.4K Views
•
09:45 min
•
February 25th, 2019
DOI :
February 25th, 2019
•Max Z. Levine*1,2, Nicole E. Gregorio*2,3, Michael C. Jewett4,5,6,7, Katharine R. Watts2,3, Javin P. Oza2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
Chapters in this video
0:04
Title
0:50
Cell Growth
3:30
Crude Cell Extract Preparation
5:30
Cell-Free Protein Synthesis Batch Format Reactions
6:25
Quantification of the Reporter Protein, [sfGFP]
7:24
Results: Modification and Optimization of the Cell-Free Platform Conditions
9:01
Conclusion
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