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Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

54.4K Views

10:31 min

July 24th, 2018

DOI :

10.3791/57932-v

July 24th, 2018


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Microbial Communities

Chapters in this video

0:04

Title

0:36

Plant Excavation, Soil Removal, and Bulk Soil Collection

1:46

Root and Rhizosphere Collection

2:44

Root Surface Sterilization

3:54

Processing Rhizosphere Samples

5:02

DNA Extraction and Soil Analysis Processing

6:33

DNA Extraction in 96-well Format

7:45

Rhizosphere Sample Loading

8:47

Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species

10:03

Conclusion

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