Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
17.5K Views
•
09:17 min
•
August 2nd, 2018
DOI :
August 2nd, 2018
•Xiaofeng Zheng1,2, Calvin Qing Wei Ho1, Xiaowei Zheng3, Kian Leong Lee4, Katarina Gradin5, Teresa S. Pereira3, Per-Olof Berggren1,2,3, Yusuf Ali1,2
1Lee Kong Chian School of Medicine, Nanyang Technological University, 2Singapore Eye Research Institute (SERI), Singapore General Hospital, 3The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, 4Cancer and Stem Cell Biology Program, Duke-NUS Medical School, 5Department of Cell and Molecular Biology, Karolinska Institutet
Chapters in this video
0:04
Title
0:33
Induction of Hypoxia in HEK293A Cells
1:28
Harvesting Cells Cultured Under Hypoxic Conditions
3:30
Preparing Nuclear Extracts
5:52
Immunoprecipitation of Proteins
7:55
Results: HIF-1α Interacts with ARNT at Hypoxia
8:56
Conclusion
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