Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
9.2K Views
•
13:47 min
•
June 3rd, 2018
DOI :
June 3rd, 2018
•Caleb Sutherland*1, Yu Wang*2, Robert V. Brown*1, Julie Foley2, Beth Mahler2, Kyathanahalli S. Janardhan2,3, Ramesh C. Kovi2,4, Anton M. Jetten1
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research, National Institute of Environmental Health Sciences, NIH, 2Cellular and Molecular Pathology Branch, Division of the National Toxicology Program, National Institute of Environmental Health Sciences, NIH, 3Integrated Laboratory Systems Inc., 4Experimental Pathology Laboratories Inc.
Chapters in this video
0:05
Title
1:00
Frozen Section Preparation for Laser Microdissection
4:25
Polyethylene Terephthalate (PET) Membrane Slides Processing and Staining Protocol
6:14
Laser Microdissection with UV Laser
11:01
Results: Evaluation of RNA Quality and Downstream RNA Analysis
12:25
Conclusion
Related Videos
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
17.3K Views
05:30
Mouse Eye Enucleation for Remote High-throughput Phenotyping
20.2K Views
16:34
Laser Capture Microdissection of Mammalian Tissue
17.0K Views
10:06
Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
14.8K Views
13:32
Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury
21.2K Views
09:20
Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
7.0K Views
15:59
Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
11.1K Views
06:53
A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
5.3K Views
09:56
In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
9.1K Views
09:09
Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion
6.8K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved