Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
14.4K Views
•
09:32 min
•
February 8th, 2018
DOI :
February 8th, 2018
•Clémence Granier1, Emeline Vinatier2,3,4, Elia Colin2,3, Marion Mandavit2,3, Charles Dariane2,3,5, Virginie Verkarre6, Lucie Biard7, Rami El Zein2, Corinne Lesaffre2, Isabelle Galy-Fauroux2, Hélène Roussel2,3,6, Eléonore De Guillebon8, Charlotte Blanc2,3, Antonin Saldmann4, Cécile Badoual2,6, Alain Gey*4, Éric Tartour*2,4
1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou
Chapters in this video
0:04
Title
0:59
Immunostaining
2:56
Slide Scanning
4:30
Image Analysis
7:16
Results: Representative Intratumoral PD-1+Tim-3+CD8+ T cell Quantification and Analysis
8:51
Conclusion
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