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The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

9.5K Views

10:30 min

October 3rd, 2017

DOI :

10.3791/56101-v

October 3rd, 2017


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C Elegans

Chapters in this video

0:05

Title

1:02

Introduction to the Accompanying Publication

2:28

RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension

3:29

Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy

5:20

Imaging the C. elegans Excretory Canal using Confocal Microscopy

8:16

Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes

9:51

Conclusion

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